ANKRD54 preferentially selects Bruton’s Tyrosine Kinase (BTK) from a Human Src-Homology 3 (SH3) domain library
نویسندگان
چکیده
Bruton's Tyrosine Kinase (BTK) is a cytoplasmic protein tyrosine kinase with a fundamental role in B-lymphocyte development and activation. The nucleocytoplasmic shuttling of BTK is specifically modulated by the Ankyrin Repeat Domain 54 (ANKRD54) protein and the interaction is known to be exclusively SH3-dependent. To identify the spectrum of the ANKRD54 SH3-interactome, we applied phage-display screening of a library containing all the 296 human SH3 domains. The BTK-SH3 domain was the prime interactor. Quantitative western blotting analysis demonstrated the accuracy of the screening procedure. Revealing the spectrum and specificity of ANKRD54-interactome is a critical step toward functional analysis in cells and tissues.
منابع مشابه
Structure and function of the SH3 domain from Bruton ́s tyrosine kinase
Mutations in the gene coding for Bruton ́s tyrosine kinase (Btk) lead to a lymphocyte differentiation block, which results in an extreme deficiency of B cells and plasma cells in the blood. These mutations are one cause of the hereditary immunodeficiency Xlinked agammaglobulinemia. Evolutionarily, Btk belongs to the Tec family of nonreceptor protein tyrosine kinases. Members of this family share...
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